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Restriction enzyme map

Purpose of this page

This web page is designed to find which restriction enzymes cut a sequence and then to identify where they cut the sequence.


After each step press one of the buttons at the bottom of the page (labelled Step 1 to 4) to move between each step. Steps 1 to 3 can be omitted or completed in any order. Finally enter your sequence and press the submit button. The restriction map is shown in the text box followed by a list of restriction enzymes that cut the sequence then those that do not.

To import a test sequence press .

Step one

Select what kind of restriction enzymes you wish to include:




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